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Research
99%+ Purity

Follistatin

Follistatin 344

Published: December 19, 2025 Last updated: January 9, 2026 Reviewed by MVP Peptides Editorial Team

Follistatin is a glycoprotein that inhibits myostatin, a protein that limits muscle growth. By blocking myostatin, follistatin may allow for increased muscle development. It has generated significant interest in muscle-wasting conditions research.

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Purity

99%+

Molecular Weight

~36 kDa (protein)

Administration

Subcutaneous injection

Storage

Store at -20°C

Mechanism of Action

Follistatin binds to and neutralizes members of the TGF-β superfamily, particularly myostatin and activin. By inhibiting these negative regulators of muscle growth, follistatin creates an environment favorable for muscle hypertrophy.

Sequence:

N/A (315 amino acid glycoprotein — too large to display as linear sequence)

Chemical Structure

Chemical structure of Follistatin

Research Areas

  • Muscular dystrophy
  • Muscle wasting
  • Sarcopenia
  • Athletic performance
  • Regenerative medicine

Potential Benefits

  • Myostatin inhibition
  • Enhanced muscle growth
  • Potential for muscle diseases
  • Improved strength
  • Research applications

Research Dosing Guidelines

For research purposes only. Not for human consumption.

Typical Dose

100-300 mcg

Frequency

Once daily

Duration

10-30 days

Administration

Subcutaneous injection

Research peptide with limited human data. Typically used in short cycles.

Reconstitution Calculator

mg
mcg
2.0 ml
0.5 ml5 ml

U-100 Insulin Syringe

0102030405060708090100

20.0 units

Concentration

500 mcg/ml

Inject Volume

0.200 ml

Syringe Units

20.0 IU

Doses Per Vial

10

For research and educational purposes only. Always follow proper reconstitution and sterile handling protocols.

Myostatin Inhibition Strategies: Follistatin in Context

Follistatin is the most potent natural myostatin inhibitor, but it's not the only approach to targeting this muscle-growth limiter. Understanding the landscape helps researchers choose the right tool.

Myostatin Inhibition Approaches

ApproachMechanismEvidence LevelAvailability
Follistatin 344Binds and neutralizes myostatin + activinStrong preclinicalResearch peptide
Follistatin gene therapyAAV-delivered follistatin overexpressionPhase I/II clinicalExperimental only
Anti-myostatin antibodiesMonoclonal antibody blockadePhase II/III clinicalClinical trials
Myostatin propeptideNatural inhibitor fragmentPreclinicalLimited availability
ACE-031 / BimagrumabActRIIB decoy receptorPhase II clinicalDiscontinued/experimental

Follistatin's advantage is its natural origin and dual inhibition of both myostatin and activin. Its limitation is protein fragility and the lack of controlled human dosing data for the injectable research peptide form.

Potential Side Effects

  • Limited human data
  • Injection site reactions
  • Theoretical concerns about uncontrolled growth

Storage Requirements

Store at -20°C. Very fragile - avoid freeze-thaw cycles.

Research References

  • [1]
    Follistatin and Muscle Regulation (2010)
    Emerging Evidence Population: Transgenic mice with follistatin overexpression

    Follistatin overexpression produced dramatic increases in skeletal muscle mass through myostatin and activin inhibition.

    Limitations: Gene therapy model; does not reflect exogenous peptide administration

  • [2]
    Inhibition of Myostatin with Emphasis on Follistatin (2009)
    Preclinical Population: Preclinical animal models and in-vitro studies

    Follistatin is the most potent natural myostatin inhibitor, capable of driving muscle growth even in myostatin-knockout models.

    Limitations: No human clinical data for exogenous follistatin administration

Frequently Asked Questions

What is Follistatin?

Follistatin is a glycoprotein that inhibits myostatin, a protein that naturally limits muscle growth, by binding to members of the TGF-β superfamily. It has generated significant research interest for its potential role in muscle-wasting conditions and regenerative medicine.

What are the potential research benefits of Follistatin?

Research suggests Follistatin may promote enhanced muscle growth by inhibiting myostatin and activin, creating an environment favorable for muscle hypertrophy. It shows particular promise in research related to muscular dystrophy, sarcopenia, and age-related muscle loss.

How is Follistatin typically dosed in research?

Follistatin is typically administered at 100-300 mcg once daily via subcutaneous injection. Research cycles are generally short, lasting 10-30 days, due to the limited availability of human dosing data for this research peptide.

What are the side effects of Follistatin?

There is limited human safety data available for Follistatin. Known potential side effects include injection site reactions, and there are theoretical concerns about uncontrolled tissue growth with prolonged use.

How should Follistatin be stored?

Follistatin should be stored at -20°C in lyophilized form and is considered very fragile. Repeated freeze-thaw cycles should be strictly avoided to prevent degradation of this large glycoprotein.

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