Peptide Reconstitution Calculator
Calculate the exact injection volume and syringe units for any peptide. Enter your vial size, water volume, and desired dose — the calculator does the rest.
Reconstitution Calculator
U-100 Insulin Syringe
10.0 units
2500 mcg/ml
0.100 ml
10.0 IU
20
For research and educational purposes only. Always follow proper reconstitution and sterile handling protocols.
How to Reconstitute Peptides
Gather Your Supplies
Lyophilized peptide vial, bacteriostatic water (BAC water), alcohol swabs, U-100 insulin syringes, and a clean workspace.
Clean the Vial Tops
Wipe the rubber stoppers of both the peptide vial and BAC water vial with alcohol swabs. Let them dry fully before proceeding.
Draw Bacteriostatic Water
Using a fresh insulin syringe, draw the desired amount of BAC water. Use the calculator above to determine the optimal volume for your vial size.
Add Water to the Peptide Vial
Insert the needle at an angle and slowly release the water down the side of the vial. Never spray directly onto the powder — this can denature the peptide.
Dissolve Gently
Swirl the vial gently in a circular motion. Do not shake. Allow 1-5 minutes for the powder to fully dissolve into a clear solution.
Calculate & Draw Your Dose
Use the calculator to find how many syringe units correspond to your desired mcg dose. Draw that amount from the vial for each injection.
Understanding Insulin Syringe Units
Peptide researchers most commonly use U-100 insulin syringes for precise dosing. Here's how to read them:
100 units
= 1.0 ml (full syringe)
50 units
= 0.5 ml (half syringe)
10 units
= 0.1 ml
Important: "Units" on an insulin syringe are not the same as "IU" (International Units) used for some hormones. In the context of peptide reconstitution, syringe units are simply a volume measurement where 100 units = 1 ml.
The Math Behind Reconstitution
Step 1: Calculate Concentration
Example: 5mg vial + 2ml BAC water = 2,500 mcg/ml
Step 2: Calculate Injection Volume
Example: 250 mcg ÷ 2,500 mcg/ml = 0.10 ml
Step 3: Convert to Syringe Units
Example: 0.10 ml × 100 = 10 units on U-100 syringe
Frequently Asked Questions
What is peptide reconstitution?
Peptide reconstitution is the process of dissolving lyophilized (freeze-dried) peptide powder with a sterile solvent, typically bacteriostatic water (BAC water). This creates a liquid solution that can be measured and administered in precise doses using an insulin syringe.
How much bacteriostatic water should I add to my peptide vial?
The amount of bacteriostatic water depends on the peptide amount in the vial and your desired concentration. A common approach is to add 2ml of BAC water to a 5mg vial, which creates a concentration of 2,500 mcg/ml. Use our calculator above to determine the exact amount for your specific vial and dosing needs.
How do I convert ml to insulin syringe units?
A standard U-100 insulin syringe holds 1ml total, divided into 100 units. Therefore, 1 unit = 0.01ml. To convert: multiply your injection volume in ml by 100 to get insulin units. For example, 0.10ml = 10 units on an insulin syringe.
How many doses can I get from one vial?
The number of doses per vial depends on the total peptide amount and your dose per injection. Divide the total peptide amount (in mcg) by your dose per injection (in mcg). For example, a 5mg vial (5,000 mcg) at 250 mcg per dose gives you 20 doses.
Does the amount of water change the peptide dose?
No. The total amount of peptide in the vial stays the same regardless of how much water you add. Adding more water simply dilutes the concentration, meaning you need to draw a larger volume per dose. Adding less water creates a more concentrated solution with a smaller injection volume per dose.
How should I store reconstituted peptides?
Once reconstituted, peptides should be stored in a refrigerator at 2-8°C (36-46°F). Most reconstituted peptides remain stable for 3-4 weeks when properly refrigerated. Never freeze reconstituted peptides, and protect them from direct light.
Learn More About Peptides
Explore our comprehensive peptide guides, safety resources, and research references.